New plant breeding techniques
Within this research theme, we aim to pioneer and refine the tools and knowledge for next-generation plant breeding by developing and studying new plant breeding techniques (NPBTs), including genome editing, cisgenesis and targeted mutagenesis. Our ambition is to deepen fundamental understanding of how these technologies can reliably and precisely alter plant genomes, thereby establishing robust scientific foundations for future breeding and functional genomics.
CRISPR-Cas and other genome editing tools
A central research focus is the development and implementation of genome editing tools, specifically CRISPR/Cas9 and related systems such as CRISPR/Cpf1 for targeted gene edits. Using these molecular “scissors,” we explore the mechanisms of gene function, test allelic variants, and evaluate the potential for allele replacement and functional diversification.
Using CRISPR-Cas we overcome genetic barriers by cutting between interesting and undesirable traits. Appealing traits often come from wild, related species. However, these are frequently linked to undesirable ones, like small, sour fruits instead of large, tasty tomatoes. Traditional methods to separate these traits depend on luck and are not easily controlled. As a result, breeders must use many plants and make numerous crosses before all interesting traits are present in the plant. This process is slow, expensive, and inefficient. With genome editing tools, we separate traits in a targeted way. As a result, plant breeding no longer needs to rely on luck, allowing the process to be accelerated.
DNA-free and transient delivery systems
The group also studies transient expression strategies, including protoplast-based systems and recombinant-DNA-free delivery. These allow genome edits without stable foreign DNA integration. Such methods are important for both fundamental research and for understanding regulatory and biosafety implications of new plant breeding techniques. They enable precise perturbations of gene activity while allowing researchers to investigate genome integrity, cellular responses and the mechanisms that govern successful editing.
Scientific and societal reflection
As part of its academic mission, the group contributes to science-based reflections on the governance and responsible use of NPBTs. This includes participation in expert groups, discussions with regulators and the preparation of position papers on techniques such as cisgenesis and genome editing. These activities ensure that scientific insights into mechanism, uncertainty and potential are translated into well-informed societal and policy dialogues.
