New breeding methods: gene editing and plant regeneration
About this expertise
In short- Gene editing (CRISPR-Cas)
- Cisgenesis
- Transformation, tissue culture & crop regeneration
- Solutions for crops that are difficult to breed
New breeding methods can contribute to plant breeding by enabling faster and more precise genetic modifications, or by introducing genes from related plants more efficiently (cisgenesis). WUR works on improving these methods—such as CRISPR-Cas—to support breeding companies in developing innovative varieties that are more resistant to diseases and abiotic stress.
WUR develops and applies modern breeding techniques that can accelerate traditional breeding processes. Examples include gene editing using CRISPR-Cas and cisgenesis. For these technologies, transformation and regeneration methods—introducing new DNA into plant cells and regenerating complete plants from single cells—are essential.
We have a proven track record in developing regeneration protocols, even for challenging crops such as pepper. Innovations such as the use of regeneration boosters and protoplast regeneration increase the applicability of DNA technologies. This enables companies to benefit from shorter development cycles, lower costs, and more robust crops.
With gene editing technologies such as CRISPR-Cas, we can make highly targeted changes to DNA. This accelerates breeding and allows desirable traits—such as disease resistance or longer shelf life—to be applied directly.
In cisgenesis, genes from the same or closely related species are introduced without adding foreign (transgenic) DNA. WUR is an international pioneer in this field, for example with disease-resistant apples and potatoes. Intragenesis involves creating new combinations of genes from within the same species.
These are indispensable for modern breeding. WUR develops tissue culture protocols for a wide range of crops, including solutions for more demanding species such as pepper and potato. Innovations such as regeneration boosters and improved protoplast regeneration enhance the usability and efficiency of these techniques.
- Disease resistance: cisgenic potatoes resistant to Phytophthora and cisgenic apples resistant to apple scab.
- Product quality: starch potatoes containing only amylopectin; improved nutritional value and extended shelf life of fruits and vegetables.
- Faster market introduction: development of new varieties through precise genetic modifications.
- Unique combination: fundamental research and practical trials in greenhouses and the field.
- Genetics & big data: identification of desired traits.
- Collaboration with companies: development of protocols, consultancy, and joint projects.
- Policy advice: input on the possibilities and implications of new breeding methods.
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Contact our expert to discuss how new breeding methods can accelerate your company’s development.
JM (Ania) Lukasiewicz
Expert new plant breeding methods